RNAi off-target prediction
Given an RNA sequence, this tool will provide a list of possible off-targets based on 19-mer sequence complementarity.
The tool first splits the RNA sequence into all possible 19-mers. These 19-mers are then matched against the full WaspAtlas transcriptome (and its reverse complement). The job report will contain information for each transcript for which at least one 19-mer matches (on-target match and possible off-targets) and the percentage of 19-mers which match. A single percentage value is returned for each WaspAtlas locus, representing the maximum percentage of 19-mers across all matched transcripts in this locus.